产品货号：
Z23032
中文名称：
PDH E1 Alpha/PDHA1抗体
英文名称：
Anti-PDH E1 Alpha/PDHA1 antibody
产品规格：
50μl|100μl|150μl
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-PDH E1 Alpha/PDHA1 antibody |
| 指标别名 | PDH E1 alpha;PDH E1α;PDHA1;PDHE1 A type I;pyruvate dehydrogenase E1 α subunit |
| 克隆性 | Polyclonal |
| 检验物种 | human,mouse,rat |
| 应用范围 | WB,IHC,ICC/IF,IP,Direct ELISA |
| 基因名称 | PDHA1 |
| 抗体来源 | Rabbit |
| 抗体类型 | IgG |
| 免疫原 | E.coli-derived human PDH E1 Alpha/PDHA1 recombinant protein (Position:R45-S390). |
| 实际分子量 | 40KD |
| 成分 | 500μg/mL antibody with PBS，0.02% NaN3,1mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500μg/mL |
| 产品形态 | 溶液 |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
| 背景资料 | The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2),and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle.The PDH complex is composed of multiple copies of three enzymatic components:pyruvate dehydrogenase (E1),dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).The E1 enzyme is a heterotetramer of two alpha and two beta subunits.This gene encodes the E1 alpha 1 subunit containing the E1 active site,and plays a key role in the function of the PDH complex.Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome.Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Uniprot ID | P08559 |
| 推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ZN1926) for Western blot,and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (ZN1865) for IHC(P) and ICC. |
| 推荐稀释比 | Western blot (WB) 1:500～2000 Immunohistochemistry in paraffin section (IHC) 1:50～400 Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50～400 ImmunoPrecipitation (IP) 1:50 Direct ELISA 1:100～1000 (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
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